Fmoc-Thr(tBu)-Wang Resin (100–200 Mesh): A Staple for Solid-Phase Peptide Synthesis

In the field of solid-phase peptide synthesis (SPPS), the right resin can define the success of a synthesis. One of the most widely used tools in peptide chemistry is Fmoc-Thr(tBu)-Wang resin, especially in the 100–200 mesh particle size range, ideal for batch and automated peptide synthesis systems.

Let’s break down why this resin is a go-to choice in modern peptide synthesis labs.

What Is Fmoc-Thr(tBu)-Wang Resin?

Fmoc-Thr(tBu)-Wang resin is a preloaded resin used for the synthesis of peptides with threonine at the C-terminus. Here's what each part of the name represents:

• Fmoc (9-fluorenylmethyloxycarbonyl): A base-labile N-terminal protecting group that allows for mild deprotection conditions.

• Thr(tBu): Threonine with a tert-butyl (tBu) group protecting the hydroxyl side chain to prevent unwanted side reactions during synthesis.

• Wang Resin: A polystyrene-based resin with a benzyl alcohol linker, ideal for producing C-terminal carboxylic acid peptides.

• 100–200 mesh: Indicates the particle size distribution—optimal for good swelling, mixing, and reagent diffusion during SPPS.

Key Benefits

• Preloaded with threonine: Saves time and ensures accurate loading for C-terminal Thr-containing peptides.

• tBu side-chain protection: Prevents side reactions and ensures the hydroxyl group is protected until the final global deprotection step.

• Wang linker compatibility: Produces peptides with a free carboxylic acid at the C-terminus—standard in many bioactive peptides.

• 100–200 mesh particle size: Balances surface area and resin handling, offering efficient coupling and washing without excessive pressure drop in automated synthesizers.

Typical Applications

• Custom peptide synthesis

• Combinatorial libraries

• Therapeutic peptide research

• Epitope mapping and SAR studies

Researchers commonly use this resin in Fmoc/tBu SPPS workflows, especially when the final peptide sequence terminates in threonine. After the synthesis, acidic cleavage (TFA) removes the tBu group and releases the peptide from the resin simultaneously.

Practical Tips

• • Swelling: Swell the resin in DMF or DCM before use to activate its full capacity.

  Loading capacity: Typically ranges from 0.4 to 1.2 mmol/g, depending on the vendor. Check specs to optimize molar equivalents.

• Cleavage protocol: Use standard TFA cocktails (e.g., TFA/TIS/H₂O) to fully remove side-chain protections and cleave from the Wang linker.

• Storage: Keep dry and store in a cool, dark place under inert gas (e.g., argon) to prevent hydrolysis or degradation.

Final Thoughts

Fmoc-Thr(tBu)-Wang resin (100–200 mesh) is a trusted building block in SPPS, offering precision, reliability, and ease of use. Whether you're synthesizing a short peptide or a complex biologic, this resin ensures a solid foundation—literally—for success in the lab.

Phone : +49 1512 4714765 ( Germany )
Phone : +1 (702) 381-3042 ( USA )
Email : [email protected]
Address : Cunostraße 56, 14193 Berlin. Germany
Address : 7373 Rowlett Park Dr, Tampa, FL 33610, United States.